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‍ Because of this, lots of people have mild to moderate vitamin B12 deficiencies due to unbalanced diets or other factors

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The retention time of the vitamin B 12 on the HPLC UV chromatogram was the same regardless of whether the mAb was treated with trypsin, endoproteinase Glu-C or without any enzyme, suggesting that the vitamin B 12 is not bound to a peptide at the time of elution (data not shown for enzyme-treated samples)