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The DDA-mode analytical conditions consisted of a full MS scan at a resolution of 70,000 and a scan range from 375 to 1500 m/z (the mass-to-charge ratio) with the automatic gain control (AGC) target value being set to 1 × 10 6 , followed by a data-dependent MS 2 mode of the 10 most intense peaks from full scan using an isolation window of 3 m/z, AGC 5 × 10 4 and a normalized collision energy of 35
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