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When the membrane potential is between Erev-F1F0-ATPase and Erev-ANT, only the function of F1F-ATP synthetase shifts and the function of ANT does not change, that is, only when the membrane potential decreases slightly, F1F0-ATP synthase can hydrolyze mitochondrial ATP without affecting the cytoplasmic ATP content (Chinopoulos, 2011)
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The resultant files were processed with El-Maven (Elucidata) alongside the Kyoto Encyclopedia of Genes and Genomes (KEGG) 20,21,22 database for metabolite assignment and manual peak integration as previously described using intact mass, isotopic patterns, and an in-house standard library 23