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Evaluation effects of nebulized gentamicin in exacerbation of chronic obstructive lung disease

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Subsequently, all samples, including the diamide-treated control and test samples, containing approximately 1 million cells each, were incubated with 250 μL of 80 μM H2DCF-DA prepared in DMSO dye in the same buffer for 20-30 minutes at 30 degree C in the dark